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Objective: To observe the clinical efficacy of acupuncture plus subtle adjustment manipulation of the cervical spine for peripheral facial paralysis.Methods: A total of 60 patients with peripheral facial paralysis who met the screening criteria were selected and divided into two groups according to the method of random number table, with 30 cases in each group. The patients in the acupuncture plus tuina group received routine treatment of acupuncture and subtle adjustment manipulation of the cervical spine, while the patients in the acupuncture group only received the same acupuncture treatment. Assessment of peripheral facial paralysis with integrated traditional Chinese and Western medicine was adopted for rehabilitation assessment. Results: The total effective rate of the acupuncture plus tuina group was 93.3％, while that of the acupuncture group was 70.0％, and there was a significant between-group difference (P<0.05). After treatment, the total scores of rehabilitation assessment in both groups were significantly higher than those before treatment (both P<0.05), and the score of the acupuncture plus tuina group was significantly higher than that of the acupuncture group (P<0.05). Conclusion: The treatment of acupuncture plus subtle adjustment manipulation of the cervical spine can improve the function of facial muscles in patients with peripheral facial paralysis, and its clinical efficacy is better than that of the acupuncture treatment alone.
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Objective To explore the magnetic resonance diffusion tensor imaging (MR-DTI) features of in the late stage of Wistar rat C6 brain glioma, and the relationship between fractional anisotropy value and tumor microarchitecture. Methods The concentration of more than 1.0 × 10
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OBJECTIVE@#To explore the magnetic resonance diffusion tensor imaging (MR-DTI) features of in the late stage of Wistar rat C6 brain glioma, and the relationship between fractional anisotropy value and tumor microarchitecture.@*METHODS@#The concentration of more than 1.0 × 10/10 μL glioma cells and complete medium were injected stereotactically into the right caudate nucleus of the experimental group (n = 35) and control group (n = 10), respectively. Conventional MRI, DTI, and enhanced T1WI scans were Performed using the GE Signa HD × 3.0T MRI scanner about 3-4 weeks after implantation for the rats. Postprocessing was done using the DTI specific software Function Tool to gain FA image. Many ROIs were drawn avoiding hemorrhage, necrosis areas in tumor parenchyma, the value of FA was recorded. Each surviving rat brain was examined histologically using HE and immunohistochemical staining for VEGF and CD34. Pearson correlation analysis was used to determine the relationships between FA values and VEGF, MVD, cell density, respectively.@*RESULTS@#A total of 35 tumor-bearing rats were confirmed the tumor formation by the subsequent MRI and pathological examination. The mean FA values of the tumor and the contralateral brain tissue were 0.17 ± 0.03 and 0.31 ± 0.05 respectively, and the difference was statistically significant (t = 12.80, P 0.05). FA value in the late stage of Wistar rat C6 brain glioma has significant positive correlation to VEGF, MVD, cell density. The correlation coefficients between FA and VEGF, MVD, and cell density were 0.67, 0.65 and 0.71 (P < 0.05), respectively.@*CONCLUSIONS@#The FA value of rat glioma tumor in the late stage can preoperatively provide an accurate, reliable and noninvasive imaging monitoring method to evaluate the microstructure of glioma (cell density, the extent of angiogenesis, fiber bundle integrity and tumor cell infiltration and so on), predict the biological behavior of the tumor and make out surgical plan.
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OBJECTIVE@#To establish stable and controllable brain injury with accurate degree and good repeatability in rat model.@*METHODS@#Controlled cortical impact (CCI) device was used to prepare for the rat brain injury model by the impact head of different model (Group A No. 4, Group B No. 5, Group C No. 6) and the impact depth (Group A: 1.5-2.0 mm, Group B: 2.5-3.0 mm, Group C: 3.5-4.0 mm) with impact time of 0.1 s and impact velocity of 2.5 m/s. Twelve rats with three months of age were used in each group (the impact depth of every two rats was added 1 mm respectively). After modeling for 1 h, magnetic resonance imaging (MRI) was received and brain histopathology was observed to assess degree of injury by model parameters of three groups.@*RESULTS@#After modeling of Group A, MRI showed that the cortex structure was damaged with a small amount of bleeding in center and mild edema around, and the total volume of injury was (28.69±4.94) mm(3). Pathology revealed the injury was confined to the superficial cortical with mild edema of nerve cell, which was assessed as mild cerebral contusion. While after modeling, MRI of Group B showed that the structure of cortex and medulla were damaged simultaneously and extended to cerebral nuclei zone, with 4 cases of hematoma in the center and larger edema range around, and the total volume of injury was (78.38±9.28) mm(3). Pathology revealed the injury range was reached nuclei zone, with swell of nerve cell and mitochondria, which was assessed to moderate cerebral contusion. After modeling of Group C, MRI showed that extensive tissue injury was appeared in cortex and medulla and deep nuclei, with 9 cases of hematoma and large edema signal of surrounding tissue T2WI, while in 5 cases, lateral nucleus of injury signal was increased, and the total volume of injury was (135.89±24.80) mm(3). Pathology revealed the deep cerebral nuclei was damaged, with the disappearance of neuronal structure and vacuolization of mitochondria, which was assessed as severe cerebral contusion. MRI changes were consistent with pathological changes in three groups of model, and the injury range was significantly different (P<0.01).@*CONCLUSIONS@#Application of CCI can make stable quantitative traumatic brain injury model, which overcomes the randomness in previous injury model and possesses highly unity in iconography and pathology changes. This can provide quantitative modeling reference for clinical research.
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Objective: To establish stable and controllable brain injury with accurate degree and good repeatability in rat model. Methods: Controlled cortical impact (CCI) device was used to prepare for the rat brain injury model by the impact head of different model (Group A No. 4, Group B No. 5, Group C No. 6) and the impact depth (Group A: 1.5-2.0 mm, Group B: 2.5-3.0 mm, Group C: 3.5-4.0 mm) with impact time of 0.1 s and impact velocity of 2.5 m/s. Twelve rats with three months of age were used in each group (the impact depth of every two rats was added 1 mm respectively). After modeling for 1 h, magnetic resonance imaging (MRI) was received and brain histopathology was observed to assess degree of injury by model parameters of three groups. Results: After modeling of Group A, MRI showed that the cortex structure was damaged with a small amount of bleeding in center and mild edema around, and the total volume of injury was (28.69±4.94) mm
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OBJECTIVE@#To discuss the role of 3D-computed tomography angiography (3D-CTA) technology in reducing injuries of large meningioma surgery.@*METHODS@#3D-CTA preoperative examinations were done in 473 patients with large meningioma (simulated group). The images were analyzed by 3D post-processing workstation. By observing the major intracranial blood vessels, venous sinus, and the compression and invasion pattern in the nerve region, assessing risk level of the surgery, simulating the surgical procedures, the surgical removal plan, surgical routes and tumor blood-supplying artery embolisation plan were performed. Two hundred and fifty seven large meningioma patients who didn't underwent 3D-CTA preoperative examination served as control group. The incidence of postoperative complications, intraoperative blood transfusion and the operation time were compared between these two groups.@*RESULTS@#Compared with the control group, the Simpson's grade I and II resection rate was 80.3% (380/473), similar with that of the control (81.3%, 209/257). The incidence of postoperative complications in 3D-CTA simulated group was 37.0% which was significantly lower than that (48.2%) of the control (P<0.01). The intraoperative blood supply for simulated group and the control was (523.4±208.1) mL and (592.0±263.3) mL, respectively, with significant difference between two groups (P<0.01). And the operation time [(314.8±106.3)] min was significantly lower in simulated group than that in the control [(358.4±147.9) min] (P<0.01).@*CONCLUSION@#Application of 3D-CTA imaging technology in risk level assessment before large-scaled meningioma resection could assist in the rational planning of tumor resectin, surgical routes, and is helpful in reducing injuries and complications and enhancing the prognosis of the patients.